Sakamoto Days follows Taro Sakamoto, the world's most feared and legendary hitman, who retires from his violent past after falling in love with Aoi, a convenience store clerk. He abandons his life of crime to marry her and have a daughter, living a peaceful life as a store owner. However, this peaceful existence is shattered when old enemies and assassins threaten his newfound family, forcing him to use his deadly skills to protect them. The story combines intense action with satirical comedy as Sakamoto navigates his dual life, protecting his loved ones from the threats of his former world.